︱Product Description    ----------------------------------------------------------------------------------------------------------

Fragmentation DNA Library Prep Kit V3 is specifically optimized for Illumina & MGI high-throughput sequencing platforms. It achieves DNA fragmentation, end repair and 3'-dA tailing in one step. The kit requires no purification and can be directly used for adapter ligation. Compared to conventional methods, Fragmentation DNA Library Prep Kit V3 offers rapid workflow, just 120 minutes from sample to library. Also, it delivers streamlined procedures, higher library conversion efficiency, broader sample compatibility and lower artifact rates. It is widely applicable to PCR library construction across diverse sample types and compatible with target enrichment workflows.

︱Product Applications    ---------------------------------------------------------------------------------------------------------

Designed for library construction on Illumina & MGI high-throughput sequencing platforms. Compatible with diverse sample types, including cfDNA/ctDNA、Genomic DNA、FFPE DNA (formalin-fixed paraffin-embedded DNA)、Microbial genomes and Long-range PCR products.

For low-complexity samples such as small genomes or long PCR products, the minimum DNA input requirement can be as low as 1 ng.

︱Data Display    -------------------------------------------------------------------------------------------------------------------

● Library preparation was performed on four samples (200ng each) using library construction products from HRK Fragmentation DNA Library Prep Kit V3, Competitor K and Competitor N, respectively. The comparative results of library concentrations are presented in Figure 1.

● Different types of DNA inputs (200ng each) were digested for 20 minutes (10 minutes for FFPE DNA). The fragment size distributions of the libraries are displayed in Figure 2.

● When gDNA input is below 500ng, both library concentration and yield increase with higher gDNA amounts, while fragment sizes show gradual enlargement. When 1μg gDNA input, the libraries demonstrate slightly lower concentration, lower yield and samller fragment size compared to those from 500ng inputs.These experimental data indicate that excessive starting template amounts can inhibit enzymatic reactions. Therefore, we recommend using no more than 500ng gDNA for library construction.

● The fragment distribution of 100ng gDNA digested for different durations and their corresponding purification ratios are shown in Figure 4. It is recommended to select the digestion time and purification ratio based on the desired library insert size. The reference guide for the enzymatic digestion time is provided in Table 1.

︱Ordering Information    --------------------------------------------------------------------------------------------------------