KAPA New products listed

KAPASeparate double-ended marker connector

（Unique Dual-Indexed Adapters，UDI）

With rigorous QC validation, the product offers up to 96 unique designs that effectively reduce label mismatches and meet multiple mixing requirements for a variety of high-throughput DNA and RNA sequencing applications:

• High-quality, ready-to-use, full-length, double-ended marker adapters that fit all of Illumina's workflows based on connecting libraries

• Unique double-ended labels, Roche's unique design, contain up to 96 non-repetitive combinations of labels to reduce the adverse effects of label mismatches

• Multiple mixed-sample sequencing from low (≥2 weights) to high (up to 96 weights) is supported for various Illumina sequencers, and both Patterned and unPatterned Flow cells are supported

• It can be used in combination with KAPA HyperPrep, Hyperplus and all KAPA RNA HyperPrep library preparation kits as well as with Roche targeted capture workflow

  KAPA The product features

• Rigorous functional verification(Based on the quality control process of real sequencing experiments and data analysis), cross-contamination between labels can be effectively blocked

• Optimized sequence design,Improve the efficiency of library construction,And significantly reduced joint dimer; At the same time, ensure that different combination tags have a consistent library conversion rate

• The 96 sub tag sequences are Roche's unique design can be used alone，Also available with customer existing tag sequencesThe joint application，To meet the needs of more mixed samples

KAPA UDIThe use of the connector effectively reduced the noise background caused by label mismatch during the multi-sample sequencing, and the contamination rate was reduced to less than 0.2% in the experiment as shown in the figure.

Each point in the dataset represents a library of whole human genome samples obtained using the PCR-free database construction process. These libraries were sequenced 8-fold (black dot) or 24-fold (blue dot) mixed samples on Illumina HiSeq X sequencers and calculated by the VeriFybamid process, reflecting the potential risk of cross-contamination of samples based on genotyping. In these contaminations, tag jumping was observed, and the implementation of this non-repeated double-ended labeling strategy significantly reduced the contamination rate of mismatch sequences when the original single-ended joint was replaced with the Kapa UDI joint. In the figure, the green line represents the average pollution rate, while the red line represents the statistical upper and lower limits (JMP analysis). Data provided by The Broad Institute (Cambridge, MA, USA).

Kapa connector reagent ordering information (please order both connector reagent and dilution buffer, such as UDI+ dilution buffer, or DI+ dilution buffer)